Abstract Purpose: To analyze gene and protein expression of metalloproteinases 1, 2, 9, 11 and 16 and their correlation with clinicopathological variables in colorectal adenocarcinoma. Methods: A retrospective study of 114 patients with colorectal adenocarcinoma treated surgically in the period 2006 to 2008 in Hospital de Câncer de Barretos - Fundação Pio XII. The evaluation of gene expression was performed by RT-PCR, and protein by immunohistochemistry. The analysis of gene expression was classified as overexpressed genes and poorly expressed (fold change of approximately 2, p<0.05). The positivity of the markers in the immunohistochemical study was performed by semi-quantitative analysis. The tissue of TMA (Tissue Microarray) was done by two independent pathologists. Results: The gene expression validated by immuno - histochemical was MMP-1(p= 0.00 and 1.57 fold change) and MMP – 2 (p= 0.01 and – 1.84 to fold change) when correlated with the histological types mucinous and adenocarcinoma NOS, MMP9 (p=0.01 and fold change of 1.13) and MMP-16 (p=0.03 and 1.61 fold change) when compared with the histological types villous and adenocarcinoma NOS, MMP - 11 statistically significant in relation to male (p = 0.04 and 1.65 fold change). Conclusions: The MMPs 1, 2, 9, 11 and 16 gene and protein expression with statistical significance in at least one of the clinicopathological variables studied. Thus, we conclude that these MMPs have potential as a prognostic factor in colorectal adenocarcinoma.

OBJETIVO: Avaliar a relação de duas proteínas que participam do mecanismo de adesão celular com o grau de diferenciação celular e os estadiamentos TNM (T: tumor, N: linfonodo, M: metástase) I e IV no câncer de cólon e reto. MÉTODOS: Foram estudados cem pacientes (54 homens e 46 mulheres) tratados por adenocarcinoma colorretal, estádios I (44) e IV (56). Os cortes histológicos do tecido tumoral foram examinados por técnica de imuno-histoquímica em relação à imunoexpressão das proteínas caderina-E e delect in colon cancer (DCC), sendo classificados como positivos quando se detectou a imunoexpressão dessas proteínas em 50% ou mais das células tumorais. RESULTADOS: Para o TNM, imunoexpressão da caderina-E estádio I: positiva em 72,7 % e negativa em 35,7% ; estádio IV: positiva em 64,3% e negativa em 35,7%. Proteína DCC: 43,2% positiva e 56,8% negativa no estádio I, e 50% positiva e 50% negativa no estádio IV. Em relação ao grau de diferenciação celular, imunoexpressão da caderina-E - GI: positiva em 70% e negativa em 30%; GII: positiva em 68,4% e 31,6% negativa; GIII: 63,6% positiva e 36,4% negativa. Imunoexpressão da DCC - GI: 40% positiva e 60% negativa; GII: 46,8% positiva e 53,2% negativa; GIII: 54,5% positiva e 45,5% negativa. Não houve diferença significativa entre os grupos. CONCLUSÃO: Os resultados dessa pesquisa permitem concluir que não há relação da imunoexpressão das proteínas caderina-E e DCC com o estadiamento TNM (I e IV) e o grau de diferenciação celular no carcinoma colorretal.

OBJECTIVE: Evaluate the relationship of two proteins, which take part in the same mechanism of cell adhesion, with the cell differentiation degree and TNM staging I and IV in colorectal cancer. METHODS: One-hundred patients (54 men and 46 women), who have received treatment for colorectal cancer, stages I (44) and IV (56), have been studied. Histological cuts of tumor tissue were examined by the immunohistochemical technique as to the expression of E-cadherin and delect in colon cancer (DCC) proteins, being classified as positive whenever it was detected immunoexpression of such proteins in 50% or more tumor cells. RESULTS: For TNM, E-cadherin immunoexpression for stage I: positive in 72.7% and negative in 35.7%; stage IV: positive in 64.3% and negative in 35.7%. For DCC protein: 43.2% positive and 56.8% negative in stage I, and 50% positive and 50% negative in stage IV. Regarding the cell differentiation degree, the immunoexpression of E-cadherin - GI: positive in 70% and negative in 30%; GII: positive in 68.4% and negative in 31.6%; GIII: positive in 63.6% and negative in 36.4%. The immunoexpression of DCC - GI: 40% positive and 60% negative; GII: 46.8% positive and 53.2% negative; GIII: 54.5% positive and 45.5% negative. There was no significant difference among groups. CONCLUSION: The results of this research make it possible to come to the conclusion that there is no relationship between the immunoexpression of E-cadherin and DCC proteins with TNM staging (I and IV) and cell differentiation degree in colorectal cancer.

OBJETIVO: Avaliar a relação de uma proteína que participa do mecanismo de adesão celular com o grau de diferenciação celular e o estadiamento TNM I e IV no CCR. MÉTODOS: Foram estudados 100 pacientes (54 homens e 46 mulheres) tratados por CCR, estádio I - 44 pacientes, estádio IV - 56 pacientes. Os cortes histológicos do tecido tumoral foram examinados por técnica de imunohistoquímica em relação à expressão da proteína caderina-E. Os cortes histológicos foram classificados como positivos ou negativos pelo método semiquantitativo. RESULTADOS: Para o TNM, expressão da caderina-E estádio I: positiva em 72,7 % e negativa em 35,7% ; estádio IV: positiva em 64,3% e negativa em 35,7%. Em relação ao grau de diferenciação celular, expressão da caderina-E; G I: positiva em 70% e negativa em 30%; G II: positiva em 68.4% e 31,6% negativa; G III: 63.6% positiva e 36,4 % negativa.. Não houve diferença significativa entre os grupos. CONCLUSÃO: Os resultados dessa pesquisa permitem concluir que não há relação da expressão da proteína caderina-E com o estadiamento TNM (I e IV) e o grau de diferenciação celular no CCR.

OBJECTIVE: To evaluate the relationship of a protein that take part in the same mechanism of cell adhesion with the cell differentiation degree and TNM staging I and IV in CRA. METHODS: One-hundred patients (54 men and 46 women), who have received treatment for CRA, stage I - 44 patients and stage IV - 56 patients, have been studied. Histological cuts of tumor tissue were examined by the immunohistochemical technique as to the expression of E-cadherin proteins. Such histological cuts were classified as positive or negative through the semi-quantitative method. RESULTS: For TNM, the E-cadherin expression for stage I: positive in 72.7% and negative in 35.7%; stage IV: positive in 64.3% and negative in 35.7%. Regarding the cell differentiation degree, the expression of E-cadherin, GI: positive in 70% and negative in 30%; GII: positive in 68.4% and negative in 31.6%; GIII: positive in 63.6% and negative in 36.4%. There was no significant difference among the groups. CONCLUSION: The results of this research come to the conclusion that there is no relationship between the expression of E-cadherin protein with TNM staging (I and IV) and cell differentiation degree in CRA.